PT期刊文章AU -帕特丽夏Barbalho盟Iscia Lopes-Cendes盟——克劳迪娅Maurer-Morelli TI -吲哚美辛增加了延迟癫痫发作和癫痫的数量减少斑马鱼癫痫模型(I6-1A) DP - 2015年4月06 TA -神经病学PG - I6-1A VI - 84 IP - 14补充4099 - //www.ez-admanager.com/content/84/14_Supplement/I6-1A.short 4100 - http://n.neurology.首页org/content/84/14_Supplement/I6-1A.full所以Neurology2015 4月06;84 AB -简介:它已经证明cyclooxygenase-2 (cox - 2)增加侮辱,癫痫发作。我们旨在调查的影响吲哚美辛pentylenetetrazole之前(PTZ)全身发作mRNA表达cox - 2基因在斑马鱼幼体的大脑。方法:在六天post-fertilization,斑马鱼是分为癫痫+吲哚美辛(SG +挪作他用;n = 5)和控制+吲哚美辛(CG +挪作他用;吲哚美辛溶液中n = 5)组和孵化24小时(110μg /毫升)。之后,动物从SG +印度受到15 mm PTZ / 20分钟,seizure-like行为和延迟癫痫发作癫痫(阶段3)进行了分析。动物从CG +挪作他用PTZ-free水处理。癫痫(SG;动物暴露在15毫米PTZ)和控制(CG)团体也调查(n = 5)。 Immediately after seizure, animals were anesthetized and their heads collected for RT-qPCR amplifications that were carried out in triplicates with ef1α as endogenous controls using TaqManTM System. The relative quantification (RQ) was calculated by the equation RQ=2 -ΔΔCT. Statistical analyses were performed by Mann-Whitney test with p<0.05. Results: The mean±SEM obtained were: (i) cox2a: SG+indo 0.5±0.06 vs SG 1.3±0.12 (p=0.004); (ii) cox2b: SG+indo 0.73±0.06 vs SG 1.73±0.18 (p=0.004). Animals pretreated with indomethacin showed longer latency to reach seizure: SG+indo: 4.6±0.33 vs SG: 2.92±0.17 (p=0.0004) and presented less number of seizure-like behavior response when compared to SG (SG+indo: 11.2±1.5 vs SG: 38.16±4.5; p=0.003). Animal Ethical Committee/UNICAMP (#3098-1). Conclusion: Indomethacin treatment prior to PTZ-induced seizure reduced the cox2a and cox2b mRNA expression levels in zebrafish brain, increased the latency to seizure onset and significantly decreased the number of seizures during PTZ exposure. Our findings support evidence that zebrafish is a valuable model for further investigations of the main role of inflammation in seizure as well as a valuable model for anti-inflammatory screening of compounds that are potentially therapeutic for seizures. Support: CEPID/BRAINN-FAPESP.Disclosure: Dr. Barbalho has nothing to disclose. Dr. Lopes-Cendes has nothing to disclose. Dr. Maurer-Morelli has nothing to disclose.Monday, April 20 2015, 1:00 pm-5:00 pm