RT期刊文章SR电子T1消炎痛增加了延迟癫痫发作和癫痫的数量减少斑马鱼癫痫模型(I6-1A)摩根富林明神经病学神经学乔FD Lippincott Williams &威尔金斯SP I6-1A VO 84 14补充A1 Patricia Barbalho A1 Iscia Lopes-Cendes A1克劳迪娅Maurer-M首页orelli年2015 UL //www.ez-admanager.com/content/84/14_Supplement/I6-1A.abstract AB简介:它已经证明cyclooxygenase-2 (cox - 2)增加一种侮辱,癫痫发作。我们旨在调查的影响吲哚美辛pentylenetetrazole之前(PTZ)全身发作mRNA表达cox - 2基因在斑马鱼幼体的大脑。方法:在六天post-fertilization,斑马鱼是分为癫痫+吲哚美辛(SG +挪作他用;n = 5)和控制+吲哚美辛(CG +挪作他用;吲哚美辛溶液中n = 5)组和孵化24小时(110μg /毫升)。之后,动物从SG +印度受到15 mm PTZ / 20分钟,seizure-like行为和延迟癫痫发作癫痫(阶段3)进行了分析。动物从CG +挪作他用PTZ-free水处理。癫痫(SG;动物暴露在15毫米PTZ)和控制(CG)团体也调查(n = 5)。后立即发作,动物麻醉和头上收集RT-qPCR放大,进行了一式三份与ef1α内生控制使用TaqManTM系统。 The relative quantification (RQ) was calculated by the equation RQ=2 -ΔΔCT. Statistical analyses were performed by Mann-Whitney test with p<0.05. Results: The mean±SEM obtained were: (i) cox2a: SG+indo 0.5±0.06 vs SG 1.3±0.12 (p=0.004); (ii) cox2b: SG+indo 0.73±0.06 vs SG 1.73±0.18 (p=0.004). Animals pretreated with indomethacin showed longer latency to reach seizure: SG+indo: 4.6±0.33 vs SG: 2.92±0.17 (p=0.0004) and presented less number of seizure-like behavior response when compared to SG (SG+indo: 11.2±1.5 vs SG: 38.16±4.5; p=0.003). Animal Ethical Committee/UNICAMP (#3098-1). Conclusion: Indomethacin treatment prior to PTZ-induced seizure reduced the cox2a and cox2b mRNA expression levels in zebrafish brain, increased the latency to seizure onset and significantly decreased the number of seizures during PTZ exposure. Our findings support evidence that zebrafish is a valuable model for further investigations of the main role of inflammation in seizure as well as a valuable model for anti-inflammatory screening of compounds that are potentially therapeutic for seizures. Support: CEPID/BRAINN-FAPESP.Disclosure: Dr. Barbalho has nothing to disclose. Dr. Lopes-Cendes has nothing to disclose. Dr. Maurer-Morelli has nothing to disclose.Monday, April 20 2015, 1:00 pm-5:00 pm