PT -期刊文章盟-亚历山大·马托斯盟-蒂亚戈Peluzzo AU -维尼Pascoal AU - Rovilson Gilioli AU - Iscia Lopes-Cendes TI -微放松管制涉及小胶质激活毛果芸香碱诱导癫痫模型(P02.217) DP - 2013年2月12日TA -神经病学PG - P02.217 P02.217 VI - 80 IP - 7补充4099 - //www.ez-admanager.com/content/80/7_Supplement/P02.217.short 4100 - http:首页//www.ez-admanager.com/content/80/7_Supplement/P02.217.full所以Neurology2013 2月12;80 AB -目的:本研究的目的是分析mir - 124的表达的急性期毛果芸香碱癫痫模型。因此,调查是否异常的小胶质激活,通常观察到在这个模型中,可以由微rna放松管制。背景:小分子核糖核酸调节基因的表达等许多重要的生物功能,是重要的发展,肿瘤形成和炎症。一微,mir - 124,是表达只在中枢神经系统,涉及维护小胶质细胞在非活性或静止状态。众所周知,活化的小胶质细胞必须正确、严格监管维护正常组织内稳态,避免组织损伤。设计/方法:我们获得的总RNA从动物的海马1、3、6和24小时,以及诱导后5天的癫痫持续状态(SE)和控制动物。我们量化表达式定量实时PCR的mir - 124。只老鼠组织获得了SE是用于我们的实验。结果:我们发现mir - 124的表达显著减少后,约为60%,相比,控制样品。 MiR-124 expression was decreased already at 1h after SE and remained low until 5 days after SE.CONCLUSIONS: Our results indicate that regulation by microRNA, especially by miR-124, can play a role in microglia activation after SE induced by pilocarpine. Thus, opening the possibility of using microRNA-based therapies in epilepsy.Supported by: FAPESP.Disclosure: Dr. Matos has nothing to disclose. Dr. Peluzzo has nothing to disclose. Dr. Pascoal has nothing to disclose. Dr. Gilioli has nothing to disclose. Dr. Lopes-Cendes has nothing to disclose.Tuesday, March 19 2013, 7:30 am-12:00 pm