TY - T1的基因表达谱基因模型广义癫痫:线索底层机制(P05.080) JF -神经学乔-神经病学SP - P05.080 LP - P05.080六世- 78 - 1补充非盟-亚历山大·马托斯盟费尼Pascoal盟Deborah Nascimento 首页AU -克莉斯婷罗查盟Jaira Vasconcellos AU -玛丽亚Chamma盟克劳迪娅Maurer-Morelli盟Almir马丁斯盟安琪拉山谷AU -安娜·露西亚戈达尔盟Iscia Lopes-Cendes Y1 - 2012/04/25 UR - //www.ez-admanager.com/content/78/1_Supplement/P05.080.abstract N2 -目的:本研究的目的是描述和比较这些菌株的基因档案通过基因表达分析。背景的纯种声音所引起的大鼠(战争)是容易声音所引起的癫痫,经过高强度的声音刺激。另一个基因模型是全身性癫痫没有发作(gea)老鼠。设计/方法:我们获得的总RNA从五个易感战争(海马和四叠体),和两个抵抗战争,以及海马的三个赫亚老鼠和三个控制纯种。基因表达分析使用GeneChip®老鼠基因组230 2.0阵列(Affymetrix™),并分析了在R环境和包Affy并从Bioconductor RankProd。过多基因本体论类别被确定与大卫软件,网络和基因相互作用和相关性与聪明才智通路分析软件。结果:丰富基因本体标识在战争中参与神经元发育和分化,调节神经元的突触传递和投影,以及信息信号。最活跃的信号通路是中道的发展(Neurod1上调),以及钙和GABA受体信号(Gabra6上调)。的主要基因在epileptogenesis微分表达式和一个可能的生物作用,Apbb1 Scn1A。赫亚老鼠我们发现差异表达的基因与中枢神经系统的发展,激活MAPK的转录因子,神经元迁移和细胞凋亡,如Nrsn1 Hspb1,安全系数,Twist1 Krt18。顶部丰富基因本体论类别包含信号肽,细胞外区域和抗原处理和表示。 Among the most activated signaling pathways were endocrine system disorders, gastrointestinal disease, cell cycle and neurosystem development, which include genes such as Prlr, Hspb1, Igfpb2, CD74 and Fos.Conclusions: Our results clearly show a different molecular signature in the two genetic rat models analyzed, which may help to clarify the underlying molecular mechanism leading to seizure predisposition in these animals.Supported by: FAPESP.Disclosure: Dr. Matos has nothing to disclose. Dr. Pascoal has nothing to disclose. Dr. Nascimento has nothing to disclose. Dr. Rocha has nothing to disclose. Dr. Vasconcellos has nothing to disclose. Dr. Chamma has nothing to disclose. Dr. Maurer-Morelli has nothing to disclose. Dr. Martins has nothing to disclose. Dr. Valle has nothing to disclose. Dr. Godard has nothing to disclose. Dr. Lopes-Cendes has nothing to disclose.Wednesday, April 25 2012, 14:00 pm-19:00 pm ER -