吲哚美辛增加了延迟癫痫发作和癫痫的数量减少斑马鱼癫痫模型(P7.001)

文摘

简介:它已经证明cyclooxygenase-2 (cox - 2)增加侮辱,癫痫发作。我们旨在调查的影响吲哚美辛pentylenetetrazole之前(PTZ)全身发作mRNA表达cox - 2基因在斑马鱼幼体的大脑。方法:在六天post-fertilization,斑马鱼是分为癫痫+吲哚美辛(SG +挪作他用;n = 5)和控制+吲哚美辛(CG +挪作他用;吲哚美辛溶液中n = 5)组和孵化24小时(110μg /毫升)。之后,动物从SG +印度受到15 mm PTZ / 20分钟,seizure-like行为和延迟癫痫发作癫痫(阶段3)进行了分析。动物从CG +挪作他用PTZ-free水处理。癫痫(SG;动物暴露在15毫米PTZ)和控制(CG)团体也调查(n = 5)。后立即发作,动物麻醉和头上收集RT-qPCR放大,进行了一式三份与ef1α内生控制使用TaqManTM系统。 The relative quantification (RQ) was calculated by the equation RQ=2 -ΔΔCT. Statistical analyses were performed by Mann-Whitney test with p<0.05. Results: The mean±SEM obtained were: (i) cox2a: SG+indo 0.5±0.06 vs SG 1.3±0.12 (p=0.004); (ii) cox2b: SG+indo 0.73±0.06 vs SG 1.73±0.18 (p=0.004). Animals pretreated with indomethacin showed longer latency to reach seizure: SG+indo: 4.6±0.33 vs SG: 2.92±0.17 (p=0.0004) and presented less number of seizure-like behavior response when compared to SG (SG+indo: 11.2±1.5 vs SG: 38.16±4.5; p=0.003). Animal Ethical Committee/UNICAMP (#3098-1). Conclusion: Indomethacin treatment prior to PTZ-induced seizure reduced the cox2a and cox2b mRNA expression levels in zebrafish brain, increased the latency to seizure onset and significantly decreased the number of seizures during PTZ exposure. Our findings support evidence that zebrafish is a valuable model for further investigations of the main role of inflammation in seizure as well as a valuable model for anti-inflammatory screening of compounds that are potentially therapeutic for seizures. Support: CEPID/BRAINN-FAPESP.

披露:Barbalho博士没有披露。Lopes-Cendes博士没有披露。Maurer-Morelli博士没有披露。